Andy Fluor™ 488 Goat Anti-Rabbit IgG (H+L) Antibody is prepared by labeling high quality goat anti-rabbit IgG (H+L) with Andy Fluor™ 488. Andy Fluor™ 488 is a superior alternative to the traditional green fluorescent dye FITC (or fluorescein) owing to its exceptional brightness and photostability.
Reconstitution and Storage: Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (50 µL for 100 µg antibody or 250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.
| Specifications | Form Information | |||||
| Host/Isotype: | Goat | Physical State: | Lyophilized power | |||
| Class: | Polyclonal | Shipping Condition: | Ambient | |||
| Type: | Secondary Antibody | Purification: | Immunoaffinity chromatography | |||
| Tested Species Reactivity: | Rabbit (Rb) | Buffer: | PBS, pH 7.4, with 0.1% BSA | |||
| Target Class: | IgG (H+L) | Preservative: | 0.02% sodium azide | |||
| Antibody Form: | Whole Antibody | Storage Conditions: | 4ºC; store in dark | |||
| Label: | Andy Fluor™ 488 | |||||
| Excitation/Emission: | 495/520nm |
|
||||
| Unit Size: | 100 µg, 500 µg | |||||
| Applications | Dilution | |||||
| Western Blot (WB) | 1:5,000 – 1:20,000 | |||||
| Immunofluorescence (IF) | 1:100 – 1:2,000 | |||||
| Immunocytochemistry (ICC) | 1:100 – 1:2,000 | |||||
| Immunohistochemistry (IHC) | 1:100 – 1:2,000 | |||||
| Flow Cytometry (FACS) | 1:100 – 1:1,000 | |||||
|
|
|
Immunofluorescent stain of α-tubulin and CCSP with Andy Fluor™ 488 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor 350 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 350 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor™ 405M Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor™ 405M Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor 430 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 430 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor 488 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 555 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 555 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor 568 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 568 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor 594 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 594 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor 647 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 647 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor 680 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 680 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor™ 750 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor™ 750 Goat Anti-Rabbit IgG (H+L) Antibody
